Journal: Journal of Cerebral Blood Flow & Metabolism
Article Title: Chronic high fat diet-induced cerebrovascular remodeling impairs recovery of blood flow after cerebral ischemia in mice
doi: 10.1177/0271678x251313723
Figure Lengend Snippet: Figure 7. HFD feeding increased inflammatory cytokine and MMP-9 activity. (a) IL-1b concentrations. (b) IL-6 concentrations. (c) A representative image of zymogram of the samples from mice fed different diets. (d) Quantification of MMP-9 activity of the samples from mice fed different diets. (e) A representative image of zymogram of the samples from mice with or without receiving IL-1b and (f) quantification of MMP-9 activity of the samples from mice with or without receiving IL-1b. Results are median with 95% confidence interval (N ¼ 6) and the data of individual animal.
Article Snippet: ELISA kits for measuring IL-1b (catalog number: MLB00C; R&D Systems) and IL-6 (catalog number: M6000B; R&D Systems) were used to quantify their content in the samples according to the manufacturer’s instructions as we described before.34 The quantity of IL-1b and IL-6 in each brain sample was standardized to the protein contents.
Techniques: Activity Assay